To bind dna, apply the sample to the qiaquick column and let sit for 1 minute. Qiaquick spin handbook en print bookmark share pdf 814kb english format file size language. Protocol qiaquick spin handbook 032001 23 qiaquick gel extraction kit protocol using a microcentrifuge this protocol is designed to extract and purify dna of 70 bp qiaquick gel extraction kit for gel the same steps as the manual qg and leads to inefficient dna binding. Optimal molar templatetoprimer ratios for manual radioactive sequencing. These are available online in convenient and compact pdf. Qiaquick spin handbook qiaquick pcr purification kit for purification of pcr products 100 bp 10 kb qiaquick nucleotide removal kit for oligonucleotide 1740mers and dna 40 bp 10 kb cleanup from enzymatic reactions qiaquick gel extraction kit for gel extraction or cleanup of dna 70 bp 10 kb from enzymatic reactions. Are qiaamp and qiaquick spin columns interchangeable. Connect the upper threaded hole of the qiavac 24 plus manifold to a vacuum source. Place a qiaquick spin column in a provided 2 ml collection tube or into a vacuum manifold. Please be sure to read the qiaquick spin handbook and the detailed qiaquick gel. Qiaprep miniprep handbook 112005 9 safety information when working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. Qiaquick gel extraction kit, for gel extraction or cleanup of dna 70 bp to 10 kb from enzymatic reactions. This protocol is designed to extract and purify dna of 70 bp.
Collection tubes are reused to reduce plastic waste. A detailed protocol for using qiaquick spin columns on the qiacube is provided with. Place a qiaquick spin column in a provided 2 ml collection tube. Qiaquick pcr purification kit protocol igem 2012 survey. For more information, please refer to the qiaquick spin handbook, which can be. Extraction of dna fragments from polyacrylamide gels using the qiaquick gel extraction kit this procedure has been adapted from the qiaquick gel extraction kit protocol and from sambrook et al.
Qiaquick spin handbook 072002 23 qiaquick gel extraction kit protocol using a microcentrifuge this protocol is designed to extract and purify dna of 70 bp to 10 kb from standard or lowmelt agarose gels in tae or tbe buffer. Qiaprep miniprep handbook 052004 9 safety information when working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. Qiaquick gel extraction kit protocol using a microcentrifuge chen. Nextgeneration whole genome sequencing of dengue virus. Elution efficiency is dependent on ph 14 label the sample as insert hand it to. It has not been thoroughly tested and optimized by qiagen.
Quiaquick nucleotide removal kit, for oligonucleotide 1740mers and dna 40 bp to 10 kb cleanup from enzymatic reactions. Purification of pcr products youngstown state university. Qiaquick pcr purification kit protocol using a microcentrifuge. The binding buffers in minelute spin kits provide the correct salt concentration. Qiaquick pcr purification kit 250, 28106 from qiagen labsave. To bind dna, apply the sample to the qiaquick column and centrifuge for 3060 s. Qiaquick spin handbook 112006 9 qiaquick kits provide high yields of pure nucleic acids, for direct use in applications such as. Qiagen qiaquick pcr purification kit, 250 rxns, 10g binding capacity, 30l elution volume, tube format, manual processing, silica technology, 100 bp to 10 kb fragment, 40mers fragments removed, ideal for sequencing, microarray analysis, ligation and transformation, restriction. Qiaamp minelute virus spin handbook 042010 7 safety information when working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles.
For departmental related matters, please visit the department of. Construc tion and charac terization of est libraries from. Ordering information product contents cat no qiacube and. Qiaquick pcr purification kit 250 from qiagen selectscience. They are not to be used for human diagnostic or drug purposes or to be administered to humans unless expressly cleared for that purpose by the food and drug administration in the usa. Minelute handbook 032008 5 storage upon arrival, open the kit and store minelute spin columns at 28c. Qiaquick gel extraction kit qiaquick spin handbook 72002. The remaining kit components can be stored at room temperature 1525c. Discard flowthrough and place the qiaquick column back into the same tube.
For more information, please refer to the qiaquick spin handbook, march 2008. Processing qiaprep, qiaquick, minelute, and rneasy spin columns on the qiavac 24 plus procedure 1. Pdf nextgeneration whole genome sequencing of dengue virus. To bind dna, apply the sample to the qiaquick column and s centrifuge for 3060 s or z apply vacuum to the manifold until all the samples have passed through the column. Purification of total dna from animal tissues spincolumn protocol. Our technical service departments are staffed by experienced scientists with extensive practical and theoretical expertise in molecular biology and the use of qiagen prod ucts. Qiagen qiaquick pcr purification kit, 250 rxns, 10g binding capacity, 30l elution volume, tube format, manual processing, silica technology, 100 bp to 10 kb fragment. For more information, please refer to the qiaquick spin handbook, march 2008, which can be found at.
Qiaquick spin handbook 032008 9 qiaquick kits provide high yields of pure nucleic acids, for direct use in applications such as. Qiaquick spin kits should be stored dry at room temperature 1525c. Minelute gel extraction kit 50 from qiagen selectscience. Under these conditions, qiaquick spin kits can be stored for up to 12 months without showing any reduction in performance and quality. Up to 400 mg agarose can be processed per spin column. Hi, is it possible to use the qiaquick pcr purification kit to purify rnacdna hybrids from lowmolecular fragments after rt without loosing the rna strand. Qiagen plasmid purification handbook 082003 3 contents kit contents 5 storage 8 technical assistance 8 product warranty and satisfaction guarantee 8 product use limitations 9 safety information 9 introduction 10 comparison of qiagen largescale plasmid purification options 10 principle and procedure. For example, add 500 l of buffer pbi to 100 l pcr sample.
This handbook is available as a downloadable pdf file from the laboratory course web page. For departmental related matters, please visit the department of molecular, cell, and developmental biology website. For longer storage, qiaquick spin kits can also be stored at 28c, but in this case the buffers should be redissolved before use. The dna binding capacity of qiaquick spin columns is tested by. Add 5 volumes of buffer pbi to 1 volume of the pcr sample and mix. Comparison of two different dna extraction methodologies. These are available online in convenient and compact pdf format. For cleanup of other enzymatic reactions, follow the protocol as described for pcr samples or use the minelute reaction. Purification of pcr products the following procedure is adapted from page 18 of the qiaquick spin handbook for the qiaquick pcr purification kit.
For more information, please consult the appropriate material safety data sheets msdss. To bind dna, apply the sample to the qiaquick column and centrifuge for 1 min or apply vacuum to the manifold until all the samples have passed through the column. For purification of up to 10 micro g pcr products, 100 bp to 10 kb. Banerjee lab ucla molecular, cell and developmental biology. Qiaquick spin handbook en print bookmark share pdf 814kb english format file size language download get adobe reader.
Jul 20, 2015 this tutorial explains how to carry out a miniprep using the qiaprep spin miniprep kit provided by qiagen. Under these conditions, minelute kits can be stored for up to 12 months without showing any reduction in performance and quality. Pcr product in reaction buffer from previous experiment. You should read through the qiaquick spin handbook posted at the 7. Minelute handbook 062004 11 adsorption to minelute membrane salt and ph dependence the minelute silicagel membrane is uniquely adapted to isolate dna from both aqueous solutions and agarose gels, and up to 5 g dna can bind to each minelute column. For more information, please refer to the qiaquick spin handbook. These are available online in convenient and compact pdf format at. Add ethanol 96100%, check cap to see if it has already been added to buffer pe before use see. Qiagen plasmid purification handbook harvard university. Qiaquick gel extraction kit protocol syracuse university. Fluorescent and radioactive sequencing ligation and transformation restriction amplification labeling in vitro transcription hybridization microinjection table 1.
Qiaquick spin handbook 032008 19 qiaquick pcr purification kit protocol using a microcentrifuge this protocol is designed to purify single or doublestranded dna fragments from pcr and other enzymatic reactions see page 8. Dna ranging from 70 bp to 10 kb is purified using a simple and fast bindwashelute procedure and an elution volume of 3050. Qiaquick gel extraction kit the qiaquick gel extraction kit cat. Qiaquick gel extraction kit protocol using a microcentrifuge. Qiagen qiaquick gel extraction kit 28704 and 28706. A detailed protocol for using qiaquick spin columns. S discard flowthrough and place the qiaquick column back in the same tube. The qiaquick gel extraction kit provides spin columns, buffers, and collection tubes for silicamembranebased purification of dna fragments from gels up to 400 mg slices or enzymatic reactions. Crisprcas9 has been explored as a transformative genome engineering tool for many eukaryotic organisms. To bind dna, apply the sample to the qiaquick column and centrifuge for. Qiaprep miniprep handbook washington state university. Insert up to 24 spin columns into the luer slots of the qiavac 24 plus.